Turnover kinetics of Photosystem I measured by the electrochromic effect in Chlorella |
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Authors: | Rene Delosme Achim Zickler Pierre Joliot |
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Affiliation: | 1. Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005, Paris France;2. Max-Volmer-Institut für Physikalische Chemie und Molekularbiologie der TU Berlin, Strasse des 17. Juni 135, 1000 Berlin 12 Germany |
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Abstract: | The rise kinetics of the absorption changes induced at 515 nm and 480 nm by a flash were studied using two types of xenon flashes of different durations. The ‘slow’ rise of the absorption change () observed by Cox and Delosme (1978 C.R. Acad. Sci. (Paris) Sér. D 282, 775–778) and Joliot P., Delosme, R. and Joliot, A. ((1977) Biochim. Biophys. Acta 459, 47–57) was found to be due to double hits occurring in the reaction centers of System I during the flash.The turnover kinetics of the reaction centers of System I after a short flash were studied by a double flash method. They are in agreement with a second order reaction between P+-700 and its electron donor. |
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Keywords: | DCMU 3-(3,4-dichlorophenyl)-1,1-dimethyl urea |
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