Isolation of a recombinant lambda phage carrying nusA and surrounding region of the Escherichia coli K-12 chromosome |
| |
| Authors: | Eugene W. Holowachuk and James D. Friesen |
| |
| Affiliation: | (1) Department of Biochemistry, University of Alberta, T6G 2H7 Edmonton, Alberta, Canada;(2) Department of Medical Genetics, University of Toronto, M5S 1A8 Toronto, Ontario, Canada |
| |
| Abstract: | Summary A recombinant bacteriophage lambda,  argG-6, has been isolated which carries the argG gene and neighbouring loci on an EcoRI-generated 15.5 Kb DNA fragment from the Escherichia coli chromosome. The locations of the argG, nusA and pnp genes on the 15.5 Kb DNA fragment have been determined. In the case of nusA, a Tn5 insertion and sub-cloning of restriction fragments were used to locate the gene. The gene products of nusA and pnp have been identified on one- and two-dimensional polyacrylamide gels. The clockwise gene order was found to be argG-nusA-pnp. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
