耐药淋球菌相关核苷酸序列的克隆与初步分析*

为克隆淋球菌染色体耐药相关核苷酸序列，我们利用抑制性消减杂交技术构建耐药性淋球菌与标准参考菌株差异DNA消减库，从中筛选淋球菌耐药相关核苷酸序列。通过初步筛选，对克隆得到的DNA片段进行测序，经GenBank和淋球菌基因组序列库检索分析，发现5个未知新核苷酸序列。这些核苷酸序列可能与淋球菌染色体耐药性相关，将为研究淋球菌耐药性提供新的实验对象。

CLONING AND IDENTIFYING ANTIBIOTIC RESISTANCE RELATIONAL GENES OF NEISSERIA GENORRHOEAE

To clone the differential genes in antibiotic resistence Neisseria gonorrhoeae,the library that contains the fragments of differential genes between antibiotic resistance strain and standard reference strain of Neisseria gonorrhoeae was constructed which used suppression subtractive hybridization technique. Then the antibiotic resistance relational genes fragments were cloned and analyzed. Antibiotic resistance Neisseria gonorrhoeae subtractive library that has high subtractive efficiency was set up successfully. The amplified library contained 2500 positive clones. Sequence analysis was performed to find the fragments of antibiotic resistance relational genes. Five sequences were unknown previously. The fragments of antibiotic resistance genes may provide an important clue for studying the mechanism of occurrence and development of antibiotic resistance Neisseria gonorrhoeae.