Complex organization of a cryptic satellite DNA in the genome of the marine invertebrate Rapana thomasiana Grosse (Gastropoda) |
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Authors: | Nedialka G Markova George G Markov |
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Institution: | Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia Bulgaria |
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Abstract: | Isopicnic centrifugation in Cs2SO4-Ag+ gradients at pH 7.0 reveals that the genome of the marine snail Rapana thomasiana Grosse (Gastropoda) contains an AT-rich satellite fraction comprising 5% of the DNA. Restriction enzyme analysis shows that the satellite DNA is composed of a number of related subsets arranged in tandem arrays. They have evolved from the segmental amplification of an 1460 bp long monomer unit with a complex inner organization. Most probably, the present basic repeat originates from an ancestral 400–500 bp long sequence in which some insertions and/or deletions have occurred. |
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Keywords: | Gene structure Satellite DNA Restriction enzyme analysis Gene amplification (Rapana thomasiana) SSC standard saline citrate solution |
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