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Determination of erythromycin concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection |
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| Authors: | Erika Hanada Hisakazu Ohtani Hajime Kotaki Yasufumi Sawada Tatsuji Iga |
| Institution: | aDepartment of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, 3-1, Hongo 7-chome, Bunkyo-ku, Tokyo 113, Japan;bFaculty of Pharmaceutical Sciences, Kyushu University, 1-1, Maidashi 3-chome, Higashi-ku, Fukuoka city, Fukuoka 812, Japan |
| Abstract: | A simple method for the quantitative determination of erythromycin (EM) concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection was developed. EM was extracted from 200 μl of plasma or liver homogenate sample under sodium hydroxide alkaline conditions with tert.-butyl methyl ether. Oleandomycin was used as an internal standard. The recovery rate of EM was up to 100%. The detector cell potential for the oxidation of EM was +1100 mV. The calibration curves were linear over the concentration ranges 0.1–20.0 μg/ml for plasma and 0.5–100.0 μg/g for liver. The method was applied to the determination of the plasma and liver concentrations of EM in rats after intravenous administration (50 mg/kg dose). The method presented here has proved to be of great use for the investigation of the pharmacokinetic characteristics of EM in small animals such as rats. |
| Keywords: | Erythromycin |
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