Regulation of liver sea bass pyruvate kinase by temperature, substrates and some metabolic effectors |
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Authors: | M D Fideu Alonso M L Perez Perez M Ruiz Amil M J Herrauz Santos |
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Abstract: | Pyruvate kinase of sea bass (Dicentrarchus labrax L.) shows positive cooperativity with respect to both substrates PEP and ADP. The temperature is a modulator of this activity, changing KS0.5 and Hill coefficient values for PEP. The enzyme shows alanine and ATP inhibition and F-1,6-P2 activation at 22 degrees C. F-1,6-P2 eliminates the effect of alanine but not that of ATP. These results could indicate a regulation of this enzyme by temperature and possess kinetic properties which are similar to that of L-type mammals. |
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