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A simple procedure for the detection of plant extracellular proteolytic enzymes |
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| Authors: | J. Stano P. Kovács I. Šafařík D. Kákoniová M. Šafaříková |
| Affiliation: | (1) Garden of Medical Plants, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic;(2) Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic;(3) Laboratory of Biochemistry and Biotechnology, Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Na sádkách 7, 370 05 České Budějovice, Czech Republic;(4) Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovak Republic |
| Abstract: | A simple procedure for the detection of extracellular plant proteolytic enzymes using insoluble dye stained gelatin substrates incorporated into an appropriate culture medium is described. Extracellular proteinases produced by the tested plant cells (callus culture and cell suspension) hydrolyzed the substrates and dyed peptide fragments were released. Dyed zones around and under the proteinase-producing callus cultures were formed on the agar medium. Similarly, coloration of the culture media using proteinase-producing cell suspensions was observed. This revised version was published online in August 2006 with corrections to the Cover Date. |
| Keywords: | extracellular proteolytic activity insoluble chromolytic substrate callus cultures |
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