| ITS作为粒毛盘菌属DNA条形码的探索 |
| |
| 引用本文： | 赵鹏,庄文颖.ITS作为粒毛盘菌属DNA条形码的探索[J].菌物学报,2011,30(6):932-937. |
| |
| 作者姓名： | 赵鹏 庄文颖 |
| |
| 作者单位： | 1. 中国科学院微生物研究所真菌学国家重点实验室(等)北京100101;中国科学院研究生院 北京100049
2. 中国科学院微生物研究所真菌学国家重点实验室(等)北京100101 |
| |
| 基金项目： | National Natural Science Foundation of China (No. 31070015) and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-EW-J-6). |
| |
| 摘 要： | 以种内与种间差异以及PCR扩增和测序成功率为评价DNA条形码的重要指标，探索ITS序列作为粒毛盘菌属DNA条形码的可行性。结果表明，ITS成功区分了研究涉及的22个种，其PCR扩增和测序成功率为100%，该片段有望成为该属区分物种的DNA条形码。
|
| 关 键 词： | 条形码间隔区 种内与种间变异 PCR扩增及测序成功率 |
Evaluation of ITS region as a possible DNA barcode for the genus Lachnum (Helotiales) |
| |
| Authors: | ZHAO Peng and ZHUANG Wen-Ying |
| |
| Institution: | State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Graduate University of Chinese Academy of Sciences, Beijing 100049, China;State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
| |
| Abstract: | The feasibility of ITS as a DNA barcode for the genus Lachnum was investigated. Intra- and inter-specific sequence divergences and easiness of sequence acquisition were considered as important criteria to estimate the barcode marker. The results revealed that ITS showed appropriate intra- and inter-specific variations, was able to separate the investigated 22 species of the genus, and was readily PCR amplified and sequenced. We suggest to use ITS as the potential DNA barcode for the genus. |
| |
| Keywords: | barcoding gap intra- and inter-specific variation PCR and sequencing success rate |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
