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| 不同染色条件对淡黄绒泡菌Physarum melleum菌核形成过程的差异显示 | |
| 引用本文： | 王晓丽,吕冬霞,李景鹏,盖玉红,于玲,李玉.不同染色条件对淡黄绒泡菌Physarum melleum菌核形成过程的差异显示[J].菌物学报,2011,30(5):808-811. |
| 作者姓名： | 王晓丽 吕冬霞 李景鹏 盖玉红 于玲 李玉 |
| 作者单位： | 1. 吉林农业大学食药用菌教育部工程研究中心 长春130118;吉林农业大学农学院 长春130118 2. 吉林农业大学食药用菌教育部工程研究中心 长春130118;佳木斯大学基础医学院 佳木斯154007 3. 吉林农业大学农学院 长春130118 4. 吉林农业大学食药用菌教育部工程研究中心 长春130118 |
| 基金项目： | 国家自然科学基金（No. 31070011）；吉林省自然科学基金（No. 201115190） |
| 摘 要： | 为了了解非细胞黏菌菌核形成过程中原质团组分及形态的变化，探索在番红-固绿二重染色、番红-焦油紫-橙黄G三重染色条件下，淡黄绒泡菌Physarum melleum菌核形成过程中原质团形态及显微结构变化的显示差异。结果表明：三重染色法可以显示原质团中纤维结构及细胞核，并且菌核随着休眠胞成熟而对焦油紫具有更强的亲和力。 |
| 关 键 词： | 黏菌 显型原质团 休眠胞 显微结构 |
Differential display of sclerotial development in Physarum melleum with different dyeing methods |
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| Authors: | WANG Xiao-Li LU Dong-Xi LI Jing-Peng GAI Yu-Hong YU Ling and LI Yu |
| Institution: | Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China College of Agronomy, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China College of Preclinical Medicine of Jiamusi University, Jiamusi 154007, China;College of Agronomy, Jilin Agricultural University, Changchun 130118, China;College of Agronomy, Jilin Agricultural University, Changchun 130118, China;College of Agronomy, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China |
| Abstract: | Display differences of patterns, microstructures and components of plasmodia were observed by using double staining of safranin O-fast green and triple staining of safranin O-cresyl violet-orange G during sclerotium development. The results showed that the nuclei and fibrous structure were visual by use of the method of triple staining, and the susceptibility of sclerotium to cresyl violet increases with dormancy cell maturation. |
| Keywords: | myxomycetes phaneroplasmodium dormancy cell microstructure |
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