Flagellin (FliC) protein sequence diversity among <Emphasis Type="Italic">Bacillus thuringiensis</Emphasis> does not correlate with H serotype diversity |
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Authors: | Brahim Soufiane Dong Xu Jean-Charles Côté |
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Institution: | (1) Research Centre, Agriculture and Agri-Food Canada, 430 Gouin Blvd, Saint-Jean-sur-Richelieu, QC, Canada, J3B 3E6 |
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Abstract: | In Escherichia coli, the fliC gene encodes flagellin, the protein responsible for eliciting the immunological reaction in H serotyping. Here, the presence
of the flagellin fliC gene was studied in 86 Bacillus thuringiensis strains encompassing 67 H serotypes. Nineteen strains from four additional species in the B. cereus sensu lato group, B. cereus, B. anthracis, B. mycoides, and B. weihenstephanensis, were added for comparison purposes. The fliC genes were amplified, cloned and their nucleotide sequences determined and translated into amino acid sequences. A bootstrapped
neighbor-joining tree was generated from the alignment of the translated amino acid sequences of the amplicons. Although most
B. thuringiensis H serotypes had different flagellin amino acid sequences, some different B. thuringiensis serovars shared identical flagellin amino acid sequences. In addition, although serovars from the same H serotype were sometimes
found clustered together, several serovars from the same H serotype carried flagellins with sufficiently different amino acid
sequences as to be located on distant clusters. No correlations could be established between flagellin (FliC) protein sequence
diversity among B. thuringiensis H serotypes and H serotype diversity. These suggest that the B. thuringiensis
fliC gene does not code for the flagellin copy responsible for eliciting the immunological reaction in H serotyping. In a previous
study, the authors have shown that the B. thuringiensis
hag gene codes for the flagellin copy responsible for eliciting the immunological reaction in H serotyping. It is proposed that
the B. thuringiensis
fliC gene studied here be renamed and that the so-called hag gene studied before be renamed fliC, both in accordance with the E. coli nomenclature.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Bacillus thuringiensis Flagellin fliC gene H serotypes |
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