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Self-compatibility in doubled haploids and their F1 hybrids,regenerated via anther culture in self-incompatible Solanum chacoense Bitt.
Authors:M Cappadocia  D S K Cheng  R Ludlum-Simonette
Institution:(1) International Plant Research Institute, 830 Bransten Road, 94070 San Carlos, CA, USA;(2) Present address: Institut Botanique de l'Université de Montréal, 4101, Rue Sherbrooke Est, H1X 2B2 Montréal, Québec, Canada;(3) Present address: Sungene Inc., Palo Alto, Calif., USA
Abstract:Summary Homozygous diploid plants originating from pollen of self-incompatible Solanum chacoense clone IP33 were analysed genetically. Among the tested individuals, two were self-compatible. As expected, all the regenerants were compatible, as pistillate parent, with the mother clone. However, three plants also displayed compatibility in the reciprocal crosses. Abnormal S-gene behaviour was observed when some androgenetic plants were intercrossed. In addition in the F1 hybrids between doubled haploids, the S-gene did not appear to function as expected, suggesting that some changes, possibly the generation of new S-alleles, had occurred.
Keywords:Anther culture  Homozygous diploids  Self-incompatibility  S-alleles
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