Enrichment of bitumen-degrading microorganisms |
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| Authors: | Christian Brunner Markus Wolf Reinhard Bachofen |
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| Affiliation: | Institute of Plant Biology, CH-8008 Zürich, Switzerland |
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| Abstract: | Enrichment cultures in a percolation apparatus with two different soil types supplemented with bitumen, were used to follow growth on this complex substrate. Microscopic techniques allowed visualisation of bacteria on solid surfaces of bitumen and soil particles; quantification was only possible in the percolation fluid. In the latter, changes in pH and amount of organic material provided evidence for metabolic activities and degradation of the substrate bitumen. |
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| Keywords: | Biodegradation Bitumen AO acridine orange CFA, carboxyfluoresceine diacetate CFU, colony forming units DAPI, 4',6'-diamidino-2-phenylindole DOC, dissolved organic carbon EB, ethidium bromide SEM, scanning electron microscopy TOC, total organic carbon |
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