Development of a novel<Emphasis Type="Italic"> Agrobacterium</Emphasis>-mediated transformation method to recover transgenic<Emphasis Type="Italic"> Brassica napus</Emphasis> plants |
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| Authors: | W?C?Wang G?Menon Email author" target="_blank">G?HansenEmail author |
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| Institution: | (1) Syngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA;(2) Present address: Diversa Corporation, 4955 Directors Place, San Diego, CA 92121, USA |
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| Abstract: | We report here an in planta method to produce transgenic Brassica napus plants. The procedure included Agrobacterium-mediated inoculation of plants at various development stages along with a vacuum infiltration step. The flowering stage appeared to be the most receptive stage for transformation and production of transgenic plants. In some cases, the flowering stage was induced either by cold treatment or by high density planting. Molecular and genetic analysis revealed that single and multiple copy events were generated and that the transgenes were transmitted to the T1 and T2 progeny in a Mendelian fashion.Abbreviations AFP Adult flowering plants - ELISA Enzyme linked immunosorbent assay - GS Germinating seedlings - GUS  -Glucuronidase - ISFP Induced small flowering plants - MS Murashige and Skoog - PPO Protoporphyrinogen oxidase - TE Tris-EDTA buffer - YEP Yeast extract-peptone mediumCommunicated by W.A. Parrott |
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| Keywords: | Agrobacterium Canola Infiltration In planta |
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