Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
| |
Authors: | Martijn F Schenk Jan HG Cordewener Antoine HP America Wendy PC van't Westende Marinus JM Smulders Luud JWJ Gilissen |
| |
Affiliation: | (1) Plant Research International, Wageningen UR, Wageningen, the Netherlands;(2) Allergy Consortium Wageningen, Wageningen UR, Wageningen, the Netherlands |
| |
Abstract: | Background Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch (Betula pendula) have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus Betula. Q-TOF LC-MSE was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |