Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen |
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| Authors: | Martijn F Schenk Jan HG Cordewener Antoine HP America Wendy PC van't Westende Marinus JM Smulders and Luud JWJ Gilissen |
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| Affiliation: | (1) Plant Research International, Wageningen UR, Wageningen, the Netherlands;(2) Allergy Consortium Wageningen, Wageningen UR, Wageningen, the Netherlands |
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| Abstract: | Background Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch (Betula pendula) have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1
and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch
species that represent the four subgenera within the genus Betula. Q-TOF LC-MSE was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances
of individual isoforms in the pollen proteome. |
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