Diversity of Novel Glutenin Subunits in Bread Wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) |
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Authors: | Abu Hena Mostafa Kamal Ki-Hyun Kim Kwang-Hyun Shin Hyung-Seok Seo Hisashi Tsujimoto Hwa-Young Heo Jong-Soon Choi Chul-Soo Park Sun-Hee Woo |
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Institution: | (1) Department of Crop Science, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk, 361-763, South Korea;(2) Laboratory of Plant Genetics and Breeding Science, Tottori University, Tottori 680-8553, Japan;(3) Breeding Resource Development, National Institute of Crop Science, Suwon, 441-857, South Korea;(4) Proteome Research Team, Korea Basic Science Institute, Daejeon, 305-333, South Korea;(5) Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, South Korea;(6) Honam Agricultural Research Institute, National Institute of Crop Science, Iksan, 570-080, South Korea; |
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Abstract: | Glutenin is a major determinant of baking performance and viscoelasticity, which are responsible for high-quality bread with
a light porous crumb structure of a well-leavened loaf. We analyzed the diversity of glutenin genes from six wheat cultivars
(Korean cvs. Keumgang and Jinpum, Chinese cvs. China-108 and Yeonnon-78, and Japanese cvs. Norin-61 and Kantou-107). Glutenins
contain two types of isoforms such as high molecular weight glutenin subunit (HMW-GS) and low molecular weight glutenin subunit
(LMW-GS). Glutenin fractions were extracted from wheat endosperm using Osborne solubility method. A total of 217 protein spots
were separated on two-dimensional gel electrophoresis with isoelectric focusing (wide range of pH 3–10). The proteins spots
were subjected to tryptic digestion and identified by matrix assisted laser desorption/ionization–time of flight mass spectrometry.
HMW-GS (43 isoforms) and LMW-GS (seven isoforms) are directly responsible for producing high-quality bread and noodles. Likewise,
all the seed storage proteins are digested to provide nutrients for the embryo during seed germination and seedling growth.
We identified the diverse glutenin subunits in wheat cultivars and compared the gluten isoforms among different wheat cultivars
according to quality. This work gives an insight on the quality improvement in wheat crop. |
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