Construction and characterization of a novel staphylokinase variant with thrombin-inhibitory activity |
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Authors: | Min Wang Yuanyuan Wang Jinfeng Wang Minji Zou Shen Liu Tao Xu Xin Cai Chen Wu Jiaxi Wang Donggang Xu |
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Institution: | (1) Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, 100850, China |
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Abstract: | Staphylokinase (SAK) is an effective thrombolysis agent for therapy of myocardial infarction. We have constructed a fusion
SAK variant (SAK-HV) with a thrombin-binding domain composed of 12 amino acids from hirudin and expressed it in Escherichia coli and purified the resultant protein. SAK-HV maintained fibrinolytic activity similar to SAK and had anticoagulant activity
attributable to its hirudin segment. Measurement of thrombin-binding activity in vitro demonstrated that SAK-HV possessed
binding activity with thrombin while SAK did not. SAK-HV might thus be a more potent thrombolytic agent with anticoagulation
property than SAK. |
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