Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture |
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Authors: | Karen M Imbalzano Iva Tatarkova Anthony N Imbalzano Jeffrey A Nickerson |
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Institution: | (1) Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA |
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Abstract: | Background MCF-10A cells are near diploid and normal human mammary epithelial cells. In three-dimensional reconstituted basement membrane
culture, they undergo a well-defined program of proliferation, differentiation, and growth arrest, forming acinar structures
that recapitulate many aspects of mammary architecture in vivo. The pre-malignant MCF-10AT cells and malignant MCF-10CA1a lines were sequentially derived from the MCF-10A parental cell
line first by expression of a constitutively active T24 H-Ras generating the MCF-10AT cell line. This was followed by repeated
selection for increasingly aggressive tumor formation from cells recovered from xenograft tumors in immuno-compromised mice,
generating the MCF-10CA1a cell line. When inoculated subcutaneously into the flanks of immuno-compromised mice, MCF-10AT cells
occasionally form tumors, whereas MCF-10CA1a cells invariably form tumors with a shorter latency than MCF-10AT derived tumors. |
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