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| 内皮祖细胞的分离培养与鉴定 | |
| 引用本文： | 白春雨,侯玲玲,庞全海,关伟军,马月辉. 内皮祖细胞的分离培养与鉴定[J]. 生物技术通报, 2010, 0(2) |
| 作者姓名： | 白春雨 侯玲玲 庞全海 关伟军 马月辉 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,北京,100194;山西农业大学动物科技学院,太古,030801 2. 北京交通大学生命科学与生物工程研究院,北京,10004, 3. 山西农业大学动物科技学院,太古,030801 4. 中国农业科学院北京畜牧兽医研究所,北京,100194 |
| 基金项目： | 国家自然科学基金资助项目，"863"课题基金资助项目，"863"课题基金资助项目 |
| 摘 要： | 内皮祖细胞的分离方法有免疫磁珠分离法、淋巴细胞分离液分离法(1.077)和差速贴壁法,这3种方法已被人们广泛使用,均可分离到一定的目的细胞。分离到的目的细胞在培养过程中逐渐分化、成熟、发育为内皮细胞。在内皮细胞和内皮祖细胞的鉴别区分,使用CD34+/CD133+/KDR+鉴定为内皮祖细胞,同时使用内皮祖细胞吞噬D il-ac-LDLFITC-UEA双阳性的方法也可鉴定为内皮祖细胞。 |
| 关 键 词： | 内皮祖细胞 分离 鉴定 |
Isolation,Culture and Identification of Endothelial Progenitor Cells |
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| Bai Chunyu,Hou Lingling,Pang Quanhai,Guan Weijun,Ma Yuehui. Isolation,Culture and Identification of Endothelial Progenitor Cells[J]. Biotechnology Bulletin, 2010, 0(2) | |
| Authors: | Bai Chunyu Hou Lingling Pang Quanhai Guan Weijun Ma Yuehui |
| Affiliation: | Bai Chunyu 1,2 Hou Lingling3 Pang Quanhai2 Guan Weijun1 Ma Yuehui1(1 Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100193,2 College of Animal Science , Veterinary Medicine,Shanxi Agriculture University,Taigu 030801,3 Institute of Life Science , Bioengineering,Beijing Jiaotong University,Beijing 100044) |
| Abstract: | There are three isolation methods of endothelial progenitor cells,including immunomagnetic beads,lymphocyte separating medium(1.077) and differential adhesion,which has been widely used and could isolate a certain number of the desired cells.These cells gradually differentiate,mature,and develope into endothelial cells in culture process.In identification,cells with CD34+/CD133+/KDR+ are identified as endothelial progenitor cells,and at the same time,the double positive phagocytosis assay of Dil-ac-LDLFITC-... |
| Keywords: | Endothelial progenitor cells Isolation Identification |
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