Structural study of fucoidan from Cladosiphon okamuranus tokida |
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Authors: | Masato Nagaoka Hideyuki Shibata Itsuko Kimura-Takagi Shusuke Hashimoto Kazumasa Kimura Takashi Makino Ritsuo Aiyama Sadao Ueyama Teruo Yokokura |
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Institution: | (1) Yakult Central Institute for Microbiological Research, Yaho 1796, Kunitachi-shi, Tokyo, 186-8650, Japan |
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Abstract: | A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone of 13-linked -fucopyranose with a half sulfate substitution at the 4-positions, and a portion of the fucose residues was O-acetylated. The data obtained from partial acid hydrolysis, a methylation analysis and NMR spectra indicated that the -glucuronic acid residue is linked to the 2-positions of the fucose residues, which were not substituted by a sulfate group. These results indicated that the average structure of this fucoidan is as follows: -(3Fuc-4(±OSO3-)1–)53GlcA12]Fuc1–]n–. (Half of each fucose residue was sulfated. One O-acetyl ester was present in every 6 fucose residues.) |
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Keywords: | fucoidan sulfated fucan seaweed |
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