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Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis
Authors:Peter J McNamara  Gregory A Bradley  J Glenn Songer
Institution:Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona 85721, USA.;Arizona Veterinary Diagnostic Laboratory, University of Arizona, Tucson, Arizona 85721, USA.;Department of Veterinary Science, University of Arizona, Tucson, Arizona 85721, USA.
Abstract:The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The with-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.
Keywords:
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