龙洞山溪鲵的血细胞组成及血红蛋白含量检测

血液学指标不仅可以反映动物生理和病理变化,也能体现动物对环境的适应。本文利用血液学方法检测了27尾龙洞山溪鲵(Batrachuperus londongensis)个体的血细胞组成和血红蛋白含量,龙洞山溪鲵血红蛋白平均含量为4.16×10~(-2) g/ml;红细胞卵圆形,细胞核椭圆形位于中央或亚中央,红细胞平均含量为6.04×10~4个/mm3;白细胞数量较少,多为圆形或近圆形,平均含量为2.90×10~3个/mm3;白细胞中淋巴细胞最多,其次为单核细胞、嗜碱性细胞和中性细胞,嗜酸性细胞最少。血液参数中仅中性细胞和嗜碱性细胞的百分比在雌雄之间存在显著差异;头体长、体重和各血液参数的相关性均不显著。同时,将研究结果与其他小鲵科动物的数据进行了比较。本文报道的龙洞山溪鲵血细胞组成和血红蛋白含量的基础数据为其后续的健康监测和诊断以及适应进化的研究提供了基础资料。

Blood Cell Composition and Hemoglobin Concentration in Batrachuperus londongensis

Study on the hematology can understand the physiological and pathological variations of animals, but also detect the adaptation of animals to the environment. However, studies on the hematology of amphibians, especially in the Urodela, are limited. Here, blood cell composition and hemoglobin concentration of Batrachuperus londongensis were researched in 27 individuals. Comparison between sexes were conducted by Independent-Samples T Test or Mann-Whitney U, and correlations between snout-vent length, body mass (Table 1) and hematological parameters were determined by linear regression, all statistical tests were performed with SPSS software. The mean of hemoglobin concentration was 4.16 × 10-2 g/ml; erythrocytes were oval with ellipsoidal nucleus that is located in the center or eccentric of the erythrocyte (Figure 1), mean erythrocyte count was 6.04 × 104 cell/mm3; the number of leucocytes was small, mean count was 2.90 × 103 cell/mm3 (Table 2). The lymphocytes were most rich leucocytes, followed by monocytes, basophils, neutrophils, and eosinophils (Table 2). Among the hematological parameters, only the percentage of neutrophils and basophils showed significantly sexual difference. Correlations between snout-vent length, body mass and hematological parameters were not significant (Table 3). Meanwhile, comparison between our results with those of other hynobiid salamanders were carried out. These basic data of blood cell componentand hemoglobin concentration reported in this study are useful in the health monitoring and diagnosis, and in understanding the evolution and adaptation of B. londongensis.