Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S |
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Authors: | Evelyn Miller Gert Wohlfarth G Diekert |
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Institution: | Institut für Mikrobiologie, Universit?t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany Tel. +49-711-6855483; Fax +49-711-6855725 e-mail: imbgd@po.uni-stuttgart.de, DE
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Abstract: | Tetrachloroethene reductive dechlorination was studied with cell extracts of a newly isolated, tetrachloroethene-utilizing
bacterium, Desulfitobacterium sp. strain PCE-S. Tetrachloroethene dehalogenase mediated the reductive dechlorination of tetrachloroethene and trichloroethene
to cis-1,2-dichloroethene with artificial electron donors such as methyl viologen. The chlorinated aromatic compounds tested so
far were not reduced. A low-potential electron donor (E
0′ < –0.4 V) was required for tetrachloroethene reduction. The enzyme in its reduced state was inactivated by propyl iodide
and reactivated by light, indicating the involvement of a corrinoid in reductive tetrachloroethene dechlorination.
Received: 28 April 1997 / Accepted: 11 July 1997 |
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Keywords: | Corrinoids Dehalospirillum multivorans Desulfitobacterium Tetrachloroethene dehalogenase Propyl iodide Reductive dechlorination Strain PCE-S Tetrachloroethene Trichloroethene |
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