| 基因枪转化法获得草地早熟禾（Poa pratensis L.）转基因植株 |
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| 引用本文： | 信金娜,韩烈保,刘君,韩秀宾. 基因枪转化法获得草地早熟禾（Poa pratensis L.）转基因植株[J]. 中国生物工程杂志, 2006, 26(8): 10-14 |
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| 作者姓名： | 信金娜 韩烈保 刘君 韩秀宾 |
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| 作者单位： | 京林业大学草坪研究所北京林业大学草坪研究所京林业大学草坪研究所 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金;国家高技术研究发展计划(863计划);教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 将与植物抗旱耐盐有关的BADH-CMO双基因、CMO单基因、DREB1A单基因三种外源基因通过基因枪法分别轰击草地早熟禾的胚性愈伤组织，经过一系列筛选与再生培养，得到了抗性植株。经过PCR、Southern杂交分析检测，证明BADH-CMO双基因、CMO基因、DREB1A基因已经成功整合到草地早熟禾的植物基因组中。
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| 关 键 词： | 草地早熟禾 BADH-CMO双基因 DREB1A基因 CMO基因 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006-05-22 |
Transformation of Kentucky bluegrasss (Poa pratensis L.) by particle bombardment |
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| XIN Jin-na,HAN lie-bao,LIU Jun,HAN Xiu-bin. Transformation of Kentucky bluegrasss (Poa pratensis L.) by particle bombardment[J]. China Biotechnology, 2006, 26(8): 10-14 |
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| Authors: | XIN Jin-na HAN lie-bao LIU Jun HAN Xiu-bin |
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| Abstract: | BADH-CMO double gene,CMO gene and DREB1A gene were transformed respectively to embryonic callus of Kentucky bluegrass by particle bombardment. Then the embryonic callas of kentucky bluegrass were put in meclium for subculture which is mixed with 100mg/L hygromycin and the meclium for plantlet regeneration which mixell with 50mg/L hy gromycin about one month. Thus,the hygromycin-selectecl plants were obtained and were transplantecl into tlowerpots. The results of the PCR and Southern blot analysis indicated that the DREB1A gene,CMO gene and BADH-CMO double-gene were integrated into the genomic DNA of Kentucky bluegrass. |
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| Keywords: | Bluegrass BADH-CMO double-gene DREB1A gene CMO gene |
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