Predominance of <Emphasis Type="Italic">Ehrlichia chaffeensis</Emphasis> in <Emphasis Type="Italic">Rhipicephalus sanguineus</Emphasis> ticks from kennel-confined dogs in Limbe,Cameroon |
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| Authors: | Lucy M Ndip Roland N Ndip Seraphine N Esemu David H Walker Jere W McBride |
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| Institution: | (1) Department of Biochemistry and Microbiology, University of Buea, P. O. Box 63, Buea, Cameroon;(2) Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa;(3) Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA;(4) Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, USA;(5) Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA |
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| Abstract: | Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes. |
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