Adaptation of the xylose fermenting yeast <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> F12 for improving ethanol production in different fed-batch SSF processes |
| |
Authors: | E Tomás-Pejó M Ballesteros J M Oliva L Olsson |
| |
Institution: | 1.CIEMAT, Biomass Unit,Madrid,Spain;2.Department of Chemical and Biological Engineering,Industrial Biotechnology, Chalmers University of Technology,G?teborg,Sweden |
| |
Abstract: | An efficient fermenting microorganism for bioethanol production from lignocellulose is highly tolerant to the inhibitors released
during pretreatment and is able to ferment efficiently both glucose and xylose. In this study, directed evolution was employed
to improve the xylose fermenting Saccharomyces cerevisiae F12 strain for bioethanol production at high substrate loading. Adapted and parental strains were compared with respect to
xylose consumption and ethanol production. Adaptation led to an evolved strain more tolerant to the toxic compounds present
in the medium. When using concentrated prehydrolysate from steam-pretreated wheat straw with high inhibitor concentration,
an improvement of 65 and 20% in xylose consumption and final ethanol concentration, respectively, were achieved using the
adapted strain. To address the need of high substrate loadings, fed-batch SSF experiments were performed and an ethanol concentration
as high as 27.4 g/l (61% of the theoretical) was obtained with 11.25% (w/w) of water insoluble solids (WIS). |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|