Glutamate-induced calcium increase in myotubes depends on up-regulation of a sodium-dependent transporter |
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Authors: | Frank Claudio Giammarioli Anna Maria Falzano Loredana Fiorentini Carla Rufini Stefano |
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Institution: | Department of Pharmacology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. |
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Abstract: | We report a study on the regulation by 2-chloro adenosine (2CA) of a glutamate (Glu) transporter in myogenic C2C12 cells. Long-term 2CA exposition significantly increased the V(max) of the Glu transporter. Moreover, 2CA-treated cells responded to Glu challenge by a rapid and transient increase in their intracellular calcium level. The above reported effects were totally abolished by treating C2C12 cells with the Na(+)-dependent Glu transporter inhibitors DL-threo-b-hydroxyaspartic acid and L-trans-pyrrolidine-2,4-dicarboxylic acid. We propose that the possible link between the Glu uptake increase and the Glu induction of calcium rise could be the depolarizing currents carried by Na(+) coupled with transporter activity. |
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