| 代谢光反应交联技术在生物大分子相互作用研究中的应用 |
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| 引用本文： | 王明超,应万涛,钱小红.代谢光反应交联技术在生物大分子相互作用研究中的应用[J].生命的化学,2014(6):779-786. |
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| 作者姓名： | 王明超 应万涛 钱小红 |
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| 作者单位： | 蛋白质组学国家重点实验室,北京蛋白质组研究中心,军事医学科学院放射与辐射医学研究所,北京102206 |
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| 基金项目： | 国家重点基础研究“973项目”（2011CB910603）; 国家高技术研究发展计划（2012AA020203）; 北京市科技新星计划（Z121107002512014） |
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| 摘 要： | 具备光反应交联活性的苯甲酮基团、叠氮苯基团以及双吖丙啶基团被广泛应用于对糖、氨基酸等代谢底物的修饰。利用生物自身的代谢机制,引入带有光反应交联基团的底物类似物,可以实现生物大分子间相互作用的原位、实时研究,从而获得更为准确的相互作用复合物的信息。本文将综述代谢光反应交联技术在聚糖-蛋白质、蛋白质-蛋白质相互作用研究中的应用进展。
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| 关 键 词： | 代谢引入 光反应交联基团 光反应交联糖类似物 光反应交联氨基酸类似物 |
Application of photocrosslinking techniques coupled with metabolic incorporation in the study of the interactions between biomacromolecules |
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| WANG Mingchao,YING Wantao,QIAN Xiaohong.Application of photocrosslinking techniques coupled with metabolic incorporation in the study of the interactions between biomacromolecules[J].Chemistry of Life,2014(6):779-786. |
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| Authors: | WANG Mingchao YING Wantao QIAN Xiaohong |
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| Institution: | (State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China) |
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| Abstract: | Photocrosslinking groups including benzophenone, aryl azides and diazirine have been used to modify the metabolic substrates such as suger and amine acid. Information of the interactions between bio- macromolecules can be accurately obtained by metabolic incorporation of these analogues following with ul- traviolet crosslinking. In this review, we will describe the recent advance on metabolic incorporation ofphoto- crosslinking analogues and the application of the technique in the study of glycan-protein and protein-protein interactions. |
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| Keywords: | metabolic incorporation photocrosslinking groups photocrosslinking suger analogue photo-crosslinking amine acid analogue |
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