Physical and functional mapping of rat-liver mitochondrial DNA |
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| Authors: | T. B. Kazakova S. G. Babich M. P. Mel'nikova N. V. Tzimbalenko E. A. Braga V. V. Nosikov |
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| Affiliation: | (1) Institute of Experimental Medicine of the USSR Academy of Medical Sciences, Leningrad, USSR;(2) Institute of Molecular Biology of the USSR Academy of Sciences, Moscow, USSR |
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| Abstract: | Summary Rat-liver mitochondrial DNA (mtDNA) contains 2 cleavage sites of the restriction endonuclease XbaI. The molecular sizes of restriction fragments are 6.6×106 and 3.7×106 D. The results of partial cleavage of mtDNA with EcoRI allow the fragment F (0.32×106 D) to be localized in the sequence ABCEGFHDA. The functional map of mtDNA is constructed for two genes of the ATP-ase mRNAs from rat-liver mitochondria. Molecular hybridization shows that the ATPase genes are located in fragment B and in the GEHD area of mtDNA EcoRI cleavage. |
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