| 一种用于流感病毒亚单位疫苗纯度分析的改进方法 |
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| 引用本文： | 田文莉,邵铭,祁骥,刘淑珍,谷堃,李长贵,杨旭,李小强.一种用于流感病毒亚单位疫苗纯度分析的改进方法[J].生物技术通讯,2013(1):98-100. |
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| 作者姓名： | 田文莉 邵铭 祁骥 刘淑珍 谷堃 李长贵 杨旭 李小强 |
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| 作者单位： | 1. 天士力金纳生物技术天津有限公司,天津 300410
2. 中国食品药品检定研究院,北京 100050 |
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| 摘 要： | 目的：在SDS-PAGE方法的基础上建立一种改进方法，用于流感病毒亚单位疫苗中间体的杂质分析。方法：用糖苷酶F对不同亚型的疫苗中间体去糖基化处理后进行SDS-PAGE，与未经去糖基化处理的还原型SDS-PAGE结果进行比较。结果：去糖基化处理消除了血凝素的糖基化对电泳迁移率的影响，从而实现了血凝素亚基和杂质的电泳分离。结论：此方法应用于流感病毒亚单位疫苗中间体的杂质分析，较之常规的还原型SDS-PAGE方法具有更高的分辨力。
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| 关 键 词： | 流感病毒亚单位疫苗 SDS-PAGE 血凝素 糖苷酶F 去糖基化 |
An Improved Method of Purity Analysis of Influenza Virus Subunit Vaccine |
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| TIAN Wen-Li,SHAO Ming,QI Ji,LIU Shu-Zhen,GU Kun,LI Chang-Gui,YANG Xu,LI Xiao-Qiang.An Improved Method of Purity Analysis of Influenza Virus Subunit Vaccine[J].Letters in Biotechnology,2013(1):98-100. |
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| Authors: | TIAN Wen-Li SHAO Ming QI Ji LIU Shu-Zhen GU Kun LI Chang-Gui YANG Xu LI Xiao-Qiang |
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| Institution: | 1.Tasly & Jenner(Tianjin) Co.,Ltd,Tianjin 300410;2.National Institutes for Food and Drug Control,Beijing 100050;China |
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| Abstract: | Objective： To establish an improved method of impurity analysis based on SDS-PAGE for the inter- mediate of influenza subunit vaccine. Methods： Different types of vaccine intermediates were deglycosylated using PNGase F followed by SDS-PAGE. Deglycosylated and non-deglycosylated samples were analyzed by SDS-PAGE. Results： Deglycosylating eliminate the effects of electrophoretic mobility by haemagglutinin glycosylation. Therefore, subunits of haemagglutinin could be separated from impurities satisfactorily by electrophoresis. Conclusion： This improved method provided a higher resolution in the impurity analysis for influenza subunit vaccine intermediate, in comparison of regular reduced SDS-PAGE. |
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| Keywords: | influenza subunit vaccine SDS-PAGE haemagglutinin PNGase F deglycosylate |
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