Identification of RAPD markers linked to the ps gene and their usefulness for purity determination of breeding lines and F1 tomato hybrids

The functional male sterility controlled by ps gene proved to be a useful tool in hybrid tomato varieties breeding in Poland. The climat conditions such as excessive temperature and high humidity have a bad effect on the expression and stability of functional male sterility. Using the RAPD methods we have identified two RAPD markers linked to the ps gene. The markers OPW 13₁₂₃₀ and OPAX 10₇₈₀ were generated by 5′CACAGCGACA 3′ and 5′CCAGGCTGAC 3′ decamers respectively in F₂ population of combination 24/29 × G-1.