Physiological and morphological study of encapsulated Saccharomyces cerevisiae |
| |
Authors: | Farid Talebnia Mohammad J Taherzadeh |
| |
Institution: | aDepartment of Chemical and Biological Engineering, Chalmers University of Technology, 412 96 Göteborg, Sweden bSchool of Engineering, University of Borås, 501 90 Borås, Sweden |
| |
Abstract: | The impact of encapsulation on the anaerobic growth pattern of S. cerevisiae CBS 8066 in a defined synthetic medium over 20 consecutive batch cultivations was investigated. In this period, the ethanol yield increased from 0.43 to 0.46 g/g, while the biomass and glycerol yields decreased by 58 and 23%, respectively. The growth rate of the encapsulated cells in the first batch was 0.13 h?1, but decreased gradually to 0.01 h?1 within the 20 sequential batch cultivations. Total RNA content of these yeast cells decreased by 39% from 90.3 to 55 mg/g, while the total protein content decreased by 24% from 460 to 350 mg/g. On the other hand, the stored carbohydrates, that is, glycogen and trehalose content, increased by factors of 4.5 and 4 within 20 batch cultivations, respectively. Higher biomass concentrations inside capsules led to a lower glucose diffusion rate through the membrane, and volumetric mass transfer coefficient for glucose was drastically decreased from 6.28 to 1.24 (cm3/min) by continuing the experiments. Most of the encapsulated yeast existed in the form of single and non-budding cells after long-term application. |
| |
Keywords: | Encapsulation Yeast physiology Ethanol Glycogen Proteins Mass transfer coefficient |
本文献已被 ScienceDirect 等数据库收录! |
|