Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp. |
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Authors: | T Ishizaki N Hirayama H Shinkawa O Nimi and Y Murooka |
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Institution: | Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan. |
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Abstract: | The nucleotide sequence of a 2.1-kilobase-pair fragment containing the Streptomyces choA gene, which codes a secreted cholesterol oxidase, was determined. A single open reading frame encodes a mature cholesterol oxidase of 504 amino acids, with a calculated Mr of 54,913. The leader peptides extend over 42 amino acids and have the characteristics of a signal sequence, including basic amino acids near the amino terminus and a hydrophobic core near the signal cleavage site. Analyses of the total amino acid composition and amino acid sequencing of the first 21 amino acids from the N terminus of the purified extracellular enzyme agree with the values deduced from nucleotide sequencing data. |
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