Lysogenization by bacteriophage lambda |
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Authors: | Philippe Kourilsky |
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Affiliation: | (1) Institut de Biologie Moléculaire, Geneva, Switzerland;(2) Département de Biologie Moléculaire, Institut Pasteur, 25, rue du Docteur Roux, F-75015 Paris, France |
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Abstract: | Summary An easy and sensitive way of measuring the proportion of E. coli cells which are lysogenized by lambda phage or lambda mutants has been devised. With this assay it was possible to analyse the lysogenic response as a function of the average phage input per cell. The results indicate that lysogenization of exponentially growing cells requires that the cells are infected by at least two phages able to replicate, or three or four phages unable to replicate. |
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