Inactivation of recombinant bacteriophage lambda by use of chemical agents and UV radiation |
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| Authors: | Clark Ewan M Wright Harry Lennon Kelly-Anne Craik Vicki A Clark Jason R March John B |
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| Institution: | BigDNA Limited, Roslin, Midlothian, Scotland, United Kingdom. |
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| Abstract: | Several approaches for the inactivation of bacteriophage lambda, including UV germicidal irradiation (UVGI) and the chemical agents Virkon-S, Chloros, Decon-90, and sodium hydroxide (NaOH), were compared. Virkon, NaOH, and UVGI caused a ≥7-log(10) reduction in phage titers. This study successfully describes several methods with potential for bacteriophage inactivation in industrial settings. |
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