慢性束缚应激大鼠中枢糖皮质激素受体变化及中药复方的影响

目的:研究慢性束缚应激时大鼠脑内糖皮质激素受体的变化以及逍遥散、四君子汤、金匮肾气丸三种中药复方对其影响.方法:制作大鼠束缚应激模型,用特制束缚架连续束缚7 d与21 d,每天3 h用免疫组织化学方法结合图像分析检测中枢(海马CA1区、齿状回、大脑皮质)糖皮质激素受体的变化.结果:慢性束缚应激后,大鼠海马CA1区、大脑皮层和齿状回GR免疫反应阳性细胞平均总面积和阳性细胞数目在慢性应激的早期(7d模型组)明显增多(P＜0.05),免疫反应强度明显增强(P＜0.01).在慢性应激的后期(21 d模型组),则表现为相关脑区GR免疫反应阳性细胞平均总面积和阳性细胞数目均明显减少(P＜0.05),免疫反应强度明显减弱(P＜0.01).中药复方各组相关脑区神经元GR免疫反应阳性细胞平均总面积和阳性细胞数目较21 d模型组明显增高,免疫反应强度明显增强,三给药组之间并无明显差异,说明三给药组均能使GR含量保持于较高的状态,同时能保持GR免疫活性,其中又以逍遥散作用为明显.结论:逍遥散、四君子汤和金匮肾气丸明显逆转糖皮质激素受体下降趋势.

Effects of Chinese herbs on glucocorticoid receptor in rat brain regions with chronic immobilization stress

Aim: To investigate the changes of glucocorticoid receptor(GR) in rat brain regions with chronic immobilization stress and the influence of Chinese herbs.Methods: We copied the rat model of chronic immobilization stress(180 min daily,repeated 7 days or 21 days),and characterized the changes of GR in hippocampus CA_1,cortex,dentate gyrus via immunohistochemistry integrated image analysis.Results: The contents of glucocorticoid receptors in hippocampus CA_1,cortex,dentate gyrus were increased and the intensity of immunity reaction was significantly stronger in the model group of 7 days than that in the normal control group(P<0.05).The contents of GR were significantly decreased and the intensity of immunity reaction was weaken in the model group of 21 days(P<(0.05)).Conclusion: Compared with the model group of 21 days,the contents of GR were significantly increased and immunity reaction was intensified in three groups of treatment with chinese herbs(P<0.05),in which the group treated with soothing-liver herbs Xiaoyaopowder was the best.