Identification of the active protein in rice bran protein having an inhibitory activity of cholesterol micellar solubility |
| |
| Authors: | Jilite Wang Masaya Shimada |
| |
| Affiliation: | Faculty of Applied Biological Sciences, Department of Applied Life Science, Gifu University, Gifu, Japan |
| |
| Abstract: | In our previous study, rice bran protein (RBP) inhibited cholesterol micellar solubility in vitro and decreased serum cholesterol level in rats. In the present study, RBP was separated and purified by size-exclusion chromatography and reversed-phase chromatography. The active protein of RBP related to cholesterol micellar solubility was identified as lectin and non-specific lipid-transfer protein 1 using MALDI-TOF mass spectrometry analysis. |
| |
| Keywords: | micelle cholesterol rice bran protein lectin non-specific lipid-transfer protein 1 |
|
