MicroRNA-34a induces transdifferentiation of glioma stem cells into vascular endothelial cells by targeting Notch pathway |
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| Authors: | Zaishun Jin Tao Zhan Jing Tao Biao Xu Huizhe Zheng Yongxia Cheng |
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| Institution: | 1. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang, People’s Republic of China;2. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People’s Republic of China;3. Department of Rehabilitation Medicine, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, People’s Republic of China;4. Department of Cardiology, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, People’s Republic of China |
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| Abstract: | The function of microRNA-34a (miR-34a) in transdifferentiation of glioma stem cells (GSCs) into vascular endothelial cells (VECs) was explored by focusing on Notch ligand Delta-like 1 (Dll1). MiR-34a mimics was transfected into CD133 + glioma cell U251. The angiogenesis feature of miR-34a transfected U251 cells was investigated and the expressions of CD31, CD34, Vwf, Notch 1, and Dll1 were quantified. Length of branching vessel-like structures in the miR-34a transfected U251 cells was significantly higher than control cells. The VEC feature of miR-34a overexpressed U251 cells was further confirmed by the expressions of CD31, CD34, and vWF. Transfection of miR-34a decreased the expression of Notch 1 and Dll1. Furthermore, the miR-34a overexpression-enhanced tube formation of GSCs was suppressed when the decreased expression of Dll1 was restored. The current study highlighted the potential of miR-34a as an inducer in GSCs’ transdifferentiation into VECs by targeting Dll1. |
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| Keywords: | delta like 1 glioma stem cells microRNA 34a Notch 1 vascular endothelial cells |
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