Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes |
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| Authors: | Toshitsugu Sato Toshikazu Irie Fumihiko Yoshino |
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| Institution: | 1. Iwate Biotechnology Research Center, Kitakami-shi, Japan;2. Department of Biotechnology and Environmental Chemistry, National University Corporation Kitami Institute of Technology, Kitami, Japan;3. Environmental Science Graduate School, The University of Shiga Prefecture, Hikone, Japan;4. Environmental Science Graduate School, The University of Shiga Prefecture, Hikone, Japan |
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| Abstract: | Lentinula edodes (shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia. In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene from Pleurotus ostreatus driven by L. edodes laccase 1 gene promoter into L. edodes for expression. The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain. |
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| Keywords: | Lentinula edodes heterologous expression manganese peroxidase lcc1 promoter |
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