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Differences in glucose yield of residues from among varieties of rice,wheat, and sorghum after dilute acid pretreatment
Authors:Hiroshi Teramura  Kengo Sasaki  Hideo Kawaguchi  Fumio Matsuda  Jun Kikuchi  Tomokazu Shirai
Institution:1. Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan;2. Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Suita, Japan;3. RIKEN Center for Sustainable Resource Science, Yokohama, Japan;4. Graduate School of Medical Life Science, Yokohama City University, Yokohama, Japan;5. Graduate School of Bioagricultural Sciences and School of Agricultural Sciences, Nagoya University, Nagoya, Japan
Abstract:Bio-refinery processes require use of the most suitable lignocellulosic biomass for enzymatic saccharification and microbial fermentation. Glucose yield from biomass solid fractions obtained after dilute sulfuric acid (1%) pretreatment (at 180 °C) was investigated using 14, 8, and 16 varieties of rice, wheat, and sorghum, respectively. Biomass solid fractions of each crop showed similar cellulose content. However, glucose yield after enzymatic hydrolysis (cellulase loading at 6.6 filter paper unit/g-biomass) was different among the varieties of each crop, indicating genotypic differences for rice, wheat, and sorghum. Nuclear magnetic resonance method revealed that the high residual level of lignin aromatic regions decreased glucose yield from solid fraction of sorghum.
Keywords:lignocellulose biomass  dilute acid pretreatment  enzymatic hydrolysis  glucose  2D NMR
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