Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles |
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Authors: | Aina Gotoh Misaki Nara Yuta Sugiyama Mikiyasu Sakanaka Hiroyuki Yachi Aya Kitakata |
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Affiliation: | 1. Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi, Japan;2. Graduate School of Biostudies, Kyoto University, Kyoto, Japan |
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Abstract: | Recently, a “human gut microbial gene catalogue,” which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the “human gut microbial gene catalogue” were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest. |
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Keywords: | gut microbes standard medium short-chain fatty acids dominant human gut bacteria Gifu anaerobic medium |
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