Dimethyl sulfoxide: A solvent for cytokinins in theAmaranthus bioassay |
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Authors: | M Kamínek J Luštinec |
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Institution: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 15, 166 30 Praha 6, Czechoslovakia |
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Abstract: | Dimethyl sulfoxide present in the agar medium at concentration 0.2 % (v/v) and lower does not inhibit cytokinin-induced betacyanin
synthesis in theAmaranthus caudatus seedlings. The activity of kinetin, N6-(Δ2-isopentenyl)adenine andtrans- zeatin is the same when these cytokinins are dissolved in either water or dimethyl sulfoxide and incorporated into the medium
after autoclaving. A simple method is described which allows the cytokinin activity of slightly water-soluble and thermolabile
compounds,e.g. aromatic urea and thiourea derivatives, to be determined in theAmaranthus bioassay. |
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