Cytochemical approach to the development and behaviour of rat alveolar type II cells in culture

The development and characteristics of rat alveolar type II cells were monitored by using various cytochemical techniques. Polarized light microscopy was found useful for observing live type II cells in culture. Cells progressively lose their birefringent granules starting from 48 h of cells in culture, indicating the disappearance of the phospholipids organized lamellae in the lamellar bodies. Similar results were obtained by using an immunocytochemical approach with antibodies raised against the apoprotein component of rat surfactant. A progressive decrease in immuno-staining corresponded to the disappearance of the lamellar bodies, and birefringence. Changes in lectins binding to the cultured type II cells were also observed. Freshly isolated and one day old cultured cells could bind Macula pomifera (MPA) but not Arachis hypogaea (PA) lectins. The reverse was found in 6-7 days old cultured cells which had the ability to bind PA but not MPA the advantage of using various cytochemical techniques for studying the development of type II cells in culture is being discussed.