Protection against experimental malaria associated with AMA-1 peptide analogue structures |
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Authors: | Salazar Luz M Alba Martha P Torres Maria H Pinto Martha Cortes Ximena Torres Libardo Patarroyo Manuel E |
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Institution: | INSERM U419, IFR 26, 9 quai MONCOUSU, 44035 Cedex, Nantes, France. |
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Abstract: | We have identified, using site-directed mutagenesis, a proline located at position 13 of Baxalpha (Bax) as crucial for the maintenance of its cytosolic conformation. The substitution of this proline by a valine results in a strong binding of Bax to mitochondria and to conformational changes monitored by a decreased sensitivity of Bax to mild proteolysis and the enhancement of its oligomerization state. Deletion of the C-terminus of Bax does not modify its intracellular localization. On the other hand, the pro-apoptotic activity of Bax is enhanced by a deletion of the C-terminus in the absence of the N-terminus but is decreased in its presence. These results suggest that both extremities functionally interact to control the activity but not the subcellular localization of Bax. |
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