Protective effects of sodium orthovanadate in diabetic reticulocytes and ageing red blood cells of Wistar rats |
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Authors: | Bihari?L?Gupta Anju?Preet Email author" target="_blank">Najma?Z?BaquerEmail author |
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Institution: | (1) Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110 067 New Delhi, India |
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Abstract: | The reticulocytes and the ageing red blood cells (RBCs) namely young (Y), middle-aged (M) and old RBCs (O) of female Wistar
rats from different groups such as control animals (C), controls treated with vanadate (C + V), alloxan-induced diabetic (D),
diabetic-treated with insulin (D + I) and vanadate (D + V), were fractionated on a percoll/BSA gradient. The following enzymes
were measured-hexokinase (HK), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), glutathione-s-transferase
(GST), alanine aminotransferase ΜlaAT), aspartate aminotransferase ΜsAT) and arginase in the hemolysates of all the RBCs fractions.
Decreases in the activity of HK and AsAT by about 70%, arginase and GSH-Px by 30% in old RBCs were observed in comparison
to reticulocytes of control animals. Increases in the activity of GSSG-R by 86%, AlaAT by more than 400% and GST by 70% were
observed in old RBCs in comparison to reticulocytes of control animals.
Alloxan diabetic animals showed a further decrease in the activities of HK in Y RBCs by 37%, M RBCs by 39% and O RBCs by 32%,
GSH-Px activity in Y RBCs by 13%, M RBCs by 20% and O RBCs by 33% and GST activity in Y RBCs by 14%, M RBCs by 42% and O RBCs
by 60% in comparison to their corresponding cells of control animals. An increase in the activity of all the enzymes studied
was also observed in reticulocytes of diabetic animals in comparison to reticulocytes of controls. The GSSG-R activity was
found to be increased in Y RBCs by 49%, M RBCs by 67% and O RBCs by 64% as compared to the corresponding age-matched cells
of control animals. The activity of arginase also decreased in Y RBCs by about10%, M RBCs by 20% and O RBCs by 30% in comparison
to the age-matched cells of control animals. A decrease in the activity of AsAT in Y and M RBCs by 30%, and O RBCs by 25%
was observed in diabetic animals in comparison to the agematched cells of control animals. The activity of AlaAT was found
to be decreased by more than 10% in Y and M RBCs and 25% in O RBCs of diabetic animals in comparison to the age-matched cells
of control animals.
Insulin administration to diabetic animals reversed the altered enzyme activity to control values. Vanadate treatment also
reversed the enzyme levels except for that of GST in old cells |
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Keywords: | Ageing alloxan diabetes antioxidants arginase vanadate insulin red blood cells transaminases |
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