Sustained Expression of Human Cytomegalovirus Glycoprotein B (UL55) in the Seeds of Homozygous Rice Plants |
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Authors: | Eilleen S Tackaberry Fiona A Prior Karen Rowlandson Monika Tocchi Jelica Mehic Suzanne Porter Mike Walsh Mark R Schleiss Peter R Ganz Ravinder K Sardana Illimar Altosaar Anil K Dudani |
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Institution: | Centre for Biologics Research, Biologics and Genetic Therapies Directorate, Sir Frederick Banting Research Centre, Health Canada, 251 Sir Frederick Banting Driveway, Tunney's Pasture, A.L. 2201E, Ottawa, ON, Canada, K1A 0K9, eilleen_tackaberry@hc-sc.gc.ca. |
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Abstract: | Production of recombinant subunit vaccines in transgenic plants may be a means of reducing vaccine costs while increasing availability and safety. A plant-derived product found safe and effective for oral administration would provide additional advantages when used as a vaccine. Outstanding issues with the technology include transgene stability through successive generations and consistent bioproduction. We previously reported expression of glycoprotein B (gB) of human cytomegalovirus in seeds of transgenic tobacco. Here the goal was to determine if gB could be similarly expressed in rice, and if so, to examine expression over several plant generations. Results show that immunoreactive gB was successfully expressed in transgenic rice seeds, with sustained expression over three generations. The gB contained several neutralizing epitopes and was stable over 27 months. |
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Keywords: | CMV gB Subunit vaccine Transgenic rice seeds Molecular biopharming |
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