| 芦荟凝集素的分离、纯化和部分性质的研究 |
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| 引用本文： | 高居易,陈苒. 芦荟凝集素的分离、纯化和部分性质的研究[J]. 热带亚热带植物学报, 2000, 8(2): 177-181 |
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| 作者姓名： | 高居易 陈苒 |
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| 作者单位： | 福建师范大学生物工程学院,福建福州,350007 |
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| 摘 要： | 新鲜芦荟叶（Ａｌｏｅ ｖｅｒａ Ｌ．ｖａｒ．ｃｈｉｎｅｎｓｉｓ（Ｈａｗ．）Ｂｅｒｇｅｒ）于室温用低浓度ＮａＣｌ溶液提取。离心和透析后，经Ｎ－乙酰氨基葡萄糖０Ｓｅｐｈａｒｏｓｅ ４Ｂ亲和层析，分离纯化出芦荟凝集素（ＡＣＬ）。用ＳｅｐｈａｄｅｘＧ－１００测表观分子量为３５ＫＤ，ＳＤＳ－ＰＡＧＥ出现两条色带；染色 ；宽带和较浅的罕带。亚基分子量分别为１５ＫＤ和２０ＫＤ。能专一性凝集兔血细胞和人血红细胞
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| 关 键 词： | 芦荟 凝集素 凝集作用 分离 纯化 糖蛋白 |
| 文章编号： | 1005-3395(2000)02-0177-05 |
| 修稿时间： | 1999-09-27 |
THE ISOLATION AND PURIFICATION OF LECTIN FROM ALOE VERA L. VAR. CHINENSIS (HAW.) BERGER AND ITS CHARACTERISTICS |
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| GAO Ju-yi,CHEN Ran. THE ISOLATION AND PURIFICATION OF LECTIN FROM ALOE VERA L. VAR. CHINENSIS (HAW.) BERGER AND ITS CHARACTERISTICS[J]. Journal of Tropical and Subtropical Botany, 2000, 8(2): 177-181 |
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| Authors: | GAO Ju-yi CHEN Ran |
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| Abstract: | Lectin was extracted from leaves of Aloe vera L. var. chinensis (Haw.) Berger in a dilute NaCI solution. After centrifugation, the collected supernatant was dialysed. Pure lectin of Aloe (ACL) was isolated by N-acetylglucosandne-Sepharose 4B affinity chromatography and purified by Sephadex G-100 gel filtration chromatography. The apparent molecular weight of ACL was 35 kD. It consisted of two subunits, 15 kD and 20 kD. ACL could agglutinate rabbit erythrocytes and the human erythrocytes with groups A and O specifically, except those with groups B and AB. The activity of ACL was inhibited by D-mannose and N-acetylglucosamine. Neutral sugar content in ACL was 4.8%. |
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| Keywords: | Aloe vera var. chinensis Lectin Purification Agglutination |
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