| 外源SOD和APX基因在转基因烟草中的表达与遗传 |
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| 引用本文： | 刘飞虎,梁雪妮,周玮,刘明求. 外源SOD和APX基因在转基因烟草中的表达与遗传[J]. 植物生理学通讯, 2006, 42(5): 877-881 |
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| 作者姓名： | 刘飞虎 梁雪妮 周玮 刘明求 |
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| 作者单位： | 1. 云南大学,生命科学学院,昆明,650091
2. 云南大学,成人教育学院,昆明,650091 |
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| 摘 要： | 分析转超氧化物歧化酶基因（SOD）或抗坏血酸过氧化物酶基因（APX）烟草及其自交和杂交后代的叶片中超氧化物歧化酶（SOD）和过氧化物酶（POD）活性的结果表明：转基因烟草的SOD和POD活性在终花期最强，不同叶位叶中SOD活性差异不明显，POD活性以下部叶为最高；转基因烟草的SOD或POD活性显著高于近等基因的非转基因品系。杂交后代（F1、F2）的SOD活性能保持稳定，略高于亲本；自交后代（S1～S3）与自交亲本的SOD和POD活性相当。
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| 关 键 词： | 转基因烟草 超氧化物歧化酶 抗坏血酸过氧化物酶 外源基因 基因表达 |
| 收稿时间： | 2006-06-06 |
| 修稿时间： | 2006-09-18 |
Expression and Heredity of Exogenous SOD or APX Gene in Transgenic Tobacco (Nicotiana tabacum L.) |
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| LIU Fei-Hu,LIANG Xue-Ni,ZHOU Wei,LIU Ming-Qiu. Expression and Heredity of Exogenous SOD or APX Gene in Transgenic Tobacco (Nicotiana tabacum L.)[J]. Plant Physiology Communications, 2006, 42(5): 877-881 |
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| Authors: | LIU Fei-Hu LIANG Xue-Ni ZHOU Wei LIU Ming-Qiu |
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| Affiliation: | 1College of Life Science;2College of Adults Education, Yunnan University, Kunming 650091, China |
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| Abstract: | In this experiment,superoxide dismutase (SOD) and/or ascorbate peroxidase (APX) activities in leaves of SOD-or APX-gene transformed tobacco (Nicotiana tabacum L.) plants and their self-bred and hybridization progenies were analyzed.The transgenic tobacco had highest SOD and peroxidase (POD) activi-ties at the end-bloom stage;no significant difference in SOD activity was observed in leaves at upper,middle and base parts of a plant,but the base leaves had the highest POD activity.The transgenic tobacco had SOD and POD activities remarkably higher than the non-transgenic tobacco.The SOD activities in hybrid progenies (F1 and F2) maintained steady and were a little more than parental plants.While the SOD or POD activities of self-bred progenies (S1-S3) and their parents were similar. |
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| Keywords: | transgenic tobacco (Nicotiana tabacum L.) superoxide dismutase (SOD) ascorbate peroxidase (APX) exogenous gene gene expression |
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