Molecular cloning,chromosome mapping and characterization of the mouse CRTH2 gene,a putative member of the leukocyte chemoattractant receptor family |
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| Institution: | 1. Emergency Medicine Unit, Outpatient Department, King Chulalongkorn, Memorial Hospital, the Thai Red Cross Society, 1873, Rama 4 Rd, Patumwan, Patumwan, Bangkok 10330, Thailand;2. Emergency Medicine Unit, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, the Thai Red Cross Society, 1873, Rama 4 Rd, Patumwan, Patumwan, Bangkok 10330, Thailand;1. Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand;2. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;3. Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;4. Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand |
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| Abstract: | We have cloned the mouse CRTH2 (chemoattractant receptor-homologous molecule expressed on TH2 cells) gene encoding a putative leukocyte chemoattractant receptor, of which human homologue is expressed selectively in Th2 but not in Th1 clones among T cell clones. The deduced amino-acid sequence of mouse CRTH2 bears 77% identity with its human homologue. Phylogenetic analysis suggested that both mouse and human CRTH2 are closely related to the N-formyl peptide receptor and the C5a receptor among leukocyte chemoattractant receptors. The mouse CRTH2 gene was mapped on chromosome 19c with FISH, where no other genes for leukocyte chemoattractant receptors are mapped. RT–PCR analysis revealed that mouse CRTH2 mRNA is expressed in various cell lineages, including both hematopoietic and non-hematopoietic cell lines. Expression was also observed in liver, lung, kidney, brain, heart, thymus, and spleen. These results suggest that mouse CRTH2 functions in a variety of cells, making the effects of CRTH2 pleiotropic. |
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