|
|||||
|
|
Effects of endothelin-3 on intestinal ion transport |
|
| Authors: | L. V. Gonz lez Bosc, M. P. Majowicz, M. C. Ortiz,N. A. Vidal |
| Affiliation: | a Cátedra de Biología Celular e Histología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Junín 956, 1er piso (1113), Buenos Aires, Argentina |
| Abstract: | We investigated the effects of endothelin 3 (ET-3) on electrolyte transport in rat small intestine using a voltage clamp technique in Ussing’s chamber. ET-3 diminished potential difference (PD) and short circuit current (Isc). ET-3 did not affect PD or Isc in low Na+ and/or D-glucose-free medium. Phloridzine (an inhibitor of sodium-glucose cotransporter [SGLT1]) pretreatment abolished the effect of ET-3 on Isc. Methylene blue (a soluble guanylate cyclase inhibitor) or N-nitro-L-arginine methyl ester (a NOS inhibitor) pretreatment delayed the effect of ET-3 on PD and Isc. ET-3 enhanced NOS activity on enterocytes and systemic NO production. Then, ET-3 could inhibit SGLT1 with the participation of NO. |
| Keywords: | Endothelin 3 Small intestine Ion transport Nitric oxide SGLT1 |
| 本文献已被 ScienceDirect 等数据库收录！ | |